HIV-1-infected individuals in antiretroviral therapy react specifically with polyfunctional T-cell responses to Gag p24.

摘要: BACKGROUND Still no effective HIV-1 prophylactic or therapeutic vaccines are available. However, as the proportion of HIV-1-infected individuals on antiretroviral treatment is increasing, knowledge about residual immune response important for possible development an vaccine. METHODS In this study, magnitude, breadth, and quality HIV-1-specific T-cell in viremic (n = 19) highly active (HAART) 14) using multicolor flow cytometry were determined. RESULTS We found that magnitude breadth CD8 significantly higher than HAART (P < 0.0001 P 0.0001, respectively) functionality overall was different 0.0020). HAART, remaining responses primarily detected upon stimulation with overlapping peptides from Gag p24, integrase, Nef. The p24 more polyfunctional corresponding observed individuals. CONCLUSIONS Identification immunogenic regions also recognized by may be vaccine development. Irrespective HLA haplotype, specific within genome targeted frequently have been identified. further studies required to establish if these particular could interesting a future might limit time opportunity escape mutations.