CD8 T-cell responses in early HIV-1 infection are skewed towards high entropy peptides.

摘要: Objective: To understand the mechanisms underlying differential targeting of T-cel I responses during HIV-1 disease progression. Design: We performed a cross-sectional analysis HIV specific CD8 T-cell in peripheral blood mononuclear cells (PBMC) obtained from 21 subjects with well characterized acute or early infection and 88 chronic infection. also longitudinal five infected one whom was studied extensively over 4-year-period. Methods: PBMC were stimulated pools peptides encompassing all proteins an interferon-γ ELISpot assay. A mean entropy score calculated for each peptide genome. Results: The group preferentially targeted variable higher while towards more conserved lower predominated In followed longitudinally, to declined those increased time. subject who 4 years, epitopes Vif Nef escape occurred three these four epitopes. During phase his infection, waned associated increase breadth mainly Gag Pol Conclusion: Taken together, data demonstrate that HlV-specific T are directed but diminish frequency disease, large part due cytotoxic lymphocyte escape.