Group M-based HIV-1 Gag peptides are frequently targeted by T cells in chronically infected US and Zambian patients.

摘要: BACKGROUND The enormous sequence diversity of HIV-1 has been a major obstacle in the development globally useful vaccine for AIDS. consensus and ancestral sequence-based immunogens minimize genetic distance between contemporary isolates strains. Hence these sequences may be promising candidates HIV vaccines or serve as universal reagent set evaluating Gag-specific responses. METHODS In this study, we measured T-cell reactivity to (subtype A, B, C group M), (group M subtype B) HXB2 Gag peptides (15-mers overlapping by 11) HIV-1-infected subjects from two reference populations. We evaluated responses 43 chronically infected US 13 Zambian C) using an interferon-gamma enzyme-linked immunosorbent spot assay. RESULTS Our findings demonstrate broad cross-reactivity nearly 70% among all seven evaluated. Consensus elicited similar levels did B B-infected patients. C-infected subjects, breadth magnitude were C, peptides. CONCLUSION data that peptide pools based on M-based can used evaluate C-based immunogens.