Qualitative and quantitative study of the growth and cell surface properties of Huntington's disease fibroblasts and age-matched controls. Lack of significant differences.

摘要: Different growth-and cell surface properties of cells grown from skin biopsy samples 25 Huntington's chorea patients, 22 at risk and agematched controls were examined in a blind study. The number explants obtained each was carefully controlled. nature (epithelial or fibroblastic) the initial outgrowth scored on all mean explant after 3 weeks culture determined trypsinization. During next 15 passages, various parameters characterizing growth studied. Maximal densities rates using standard plating density passage. These also three media: DME-NCS, DME-FCS, Ham's F10 fetal calf serum (FCS). In addition, effects high temperature (40°C) addition insulin to medium protein content examined. results, containing 152 measurements 81 cases, phased multivariate analysis. Blind cluster analysis showed that clusters nonrandomly determined. Factor indicated be among most discriminating. Discriminant analysis, however, did not prove any diagnostic use, since external factors masked possible genetic difference. Two sources bias identified: size type medium. HD control fibroblasts further investigated two different techniques. adhesion single either layers normal revealed no significant differences. Intradermal immunization rabbits with whole resulted, both lines, polyspecific antiserum antibodies directed against components. When compared antisera, qualitative quantitative differences precipitation patterns crossed immunoelectrophoresis, (CIE) found. Our investigations allow us discriminate cultures by tested.