Polymorphous endocytotic organelles in the receptor-mediated endocytosis of gold-labelled alpha 2-macroglobulin complexes by human fibroblasts
作者： B. Van der Schueren , H. Van den Berghe , J.J. Cassiman , D. Gasser , F. Van Leuven
DOI:
关键词: Receptor-mediated endocytosis 、 Organelle 、 Polyclonal antibodies 、 Biology 、 Cytochemistry 、 Cell biology 、 Vesicle 、 Endocytosis 、 Endoplasmic reticulum 、 Ultrastructure
摘要: The receptor-mediated endocytosis of gold-labelled alpha 2-macroglobulin complexes with trypsin or methylamine (alpha 2M-T-Au 2M-MA-Au) was studied by electron microscopy in human skin fibroblasts. gold label found coated structures and very small tubules as well tubulovesicular multivesicular bodies/lysosomes. Thick sections (200 nm), but especially serial thin sections, clearly showed the polymorphic character cellular involved endocytosis. Numerous intercommunications were particularly obvious between structures, larger vesicles bodies (MVB). Continuities MVBs endoplasmic reticulum interconnections also observed. specificity staining reaction confirmed indirect labelling intracellular 2M polyclonal monoclonal antibodies on ultracryosections. These findings are discussed relation to observations made epithelial cells other ligands.
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