Heterophilic Binding of the Adhesion Molecules Poliovirus Receptor and Immunoglobulin Superfamily 4A in the Interaction Between Mouse Spermatogenic and Sertoli Cells1

摘要: The cell adhesion protein immunoglobulin superfamily 4A (IGSF4A) is expressed on the surfaces of spermatogenic cells in mouse testis. During spermatogenesis, IGSF4A considered to bind surface Sertoli a heterophilic manner. To identify this unknown partner IGSF4A, we generated rat monoclonal antibodies against membrane proteins grown primary culture. Using these antibodies, isolated clone that immunostained and reacted with product immunoprecipitation homogenate testis anti-IGSF4A antibody. Subsequently, recognized by antibody, performed expression cloning cDNA library from As result, identified poliovirus receptor (PVR), which another IGSF-type molecule, as binding IGSF4A. raised PVR immunoprecipitated both antigens Immunoreactivity for was present but not at all stages spermatogenesis. Overexpression TM4, line, increased more than three-fold its capacity adhere Tera-2, human line expresses These findings suggest responsible, least part, interaction between during