Epstein-Barr virus latent membrane protein 1 is essential for B-lymphocyte growth transformation.

摘要: Abstract The gene encoding latent-infection membrane protein 1 (LMP1) was specifically mutated in Epstein-Barr virus (EBV) recombinants by inserting a nonsense linker after codon 9 or 84 into an intron 186 bp 3' to the latter insertion site. EBV with LMP1 mutation were wild type for expression and growth transformation of primary B lymphocytes. In contrast, mutations open reading frame expressed N-terminally truncated crossreactive proteins could initiate maintain B-lymphocyte only when wild-type provided trans coinfecting, transformation-defective EBV, P3HR-1. These data indicate that is essential EBV-mediated lymphocytes, first 43 amino acids are critical LMP1's function, 44-initiated does not have dominant negative effect on transformation.