Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) induces inhibition of intrathymic T-cell development in hGM-CSF receptor transgenic mice.

摘要: Thymocytes show differential cytokine responses, depending on the stage of differentiation. Whether these responses are due to preferential receptor expression or downstream signaling mechanisms is unknown. In this study, we examined relationship between and T-cell proliferation differentiation using thymocytes from transgenic mice constitutively expressing human granulocyte-macrophage colony-stimulating factor (hGM-CSF) receptor. Transgenic CD4- CD8-, CD4+ CD8+ cells proliferated when cultured with hGM-CSF in vitro, whereas failed proliferate. To examine effect development, used fetal thymic organ cultures. The addition exogenous resulted failure CD8- differentiate into cells. more closely identify maturational inhibition, reconstituted normal lobes sorted pro-T-, pre-T-, post-pre-T-precursor mice. cultures led a block both pro-T- pre-T-cell differentiation, mature post-pre-T differentiated normally. We propose that during development results stage-specific inhibition precursor maturation.