Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture.

摘要: Addition of human rIL-7 to fetal thymic organ culture started at day 13, 14, or 15 did not influence the number cells generated during a 12-day period. However, IL-7 treatment resulted in preferential expansion with phenotype characteristic for an early step differentiation. The were CD4-CD8-CD3-CD2- and SCA-1+. Analysis coordinate expression CD44 CD25 on these showed that majority either CD44+CD25- CD44+CD25intermediate. TCR-alpha beta present but significantly lower as compared control cultures. cell TCR-gamma delta was increased. All effects moderate after 6 days, unequivocal 12 days culture. Treatment mAb-neutralizing murine inhibition proliferation thymocytes. No particular subset studied preferentially inhibited. By using model reconstitution 14-day embryonic thymuses depleted thymocytes by deoxyguanosine reconstituted 13 liver set up without IL-7, it shown clear cut way indeed promotes precursor cells, prevents generation cells. In addition, experiments presence IL-7. This growth inhibiting subset. These data indicate acts T differentiation plays role expand this population from additional towards pathways, whereas is influenced even enhanced.