Equilibrium analysis of [3H]TCP binding: effects of glycine, magnesium and N-methyl-D-aspartate agonists.

摘要: Abstract It has been reported that glutamate can increase the binding of [ 3 H]TCP to phencyclidine (PCP) receptors by an action on which are selective for N-methyl-D-aspartate (NMDA). Recently this laboratory glycine and magnesium amplify effect NMDA agonists in well-washed, lysed cortical membranes. Here we report maximally effective concentrations (10 μM), (300 MgCl 2 μM) affinity PCP receptor approximately 4-fold absence any change density receptors. However, combination with glutamate, had further increasing B max about 75%. Finally, a synaptosomal P preparation, not washed minimize concentration endogenous effectors value similar well-washed but K D 8-fold lower. These data indicate primary agonists, glycine, low ions is convert from low-affinity high-affinity state. discussed relation functional regulation ionophore.