Vectors with restriction-site banks. I. pJRD158, a 3903-bp plasmid containing 28 unique cloning sites.
作者： John Davison , Michel Heusterspreute , Mireille Merchez , Françoise Brunel
DOI: 10.1016/0378-1119(84)90148-3
关键词: Genetics 、 Molecular cloning 、 Cloning vector 、 Plasmid 、 Cloning 、 Molecular biology 、 Multiple cloning site 、 PBR322 、 Restriction site 、 Restriction enzyme 、 Biology
摘要: Abstract A DNA fragment has been constructed that contains many unique cloning sites not present m currently used Escherichia coli plasmid vehicles. Insertion of this into a modified version pBR322 results in an Amp R Tet vector (pJRD158) 3903 bp containing 28 sites, four “almost unique” and eight unassigned 6-bp palindromes. The the additional advantages very high copy number altered incompatibility. latter permits it to be stably maintained same host as pBR322.
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