Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

摘要: Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution resolution discussed. A time course exonuclease digest a 55 nt in transcript was analyzed investigate the performance MALDI-MS on complex mixtures. Based these data, analysis DNA sequencing reactions, produced action an polymerase, is