Intracellular Association of a Mutant Insulin-like Growth Factor Receptor with Endogenous Receptors
作者： Jin Ying Wang , Gaetano Romano , Xiao Tu , Renato Baserga , Krzysztof Reiss
DOI:
关键词: Biology 、 Growth factor receptor 、 Mechanism of action 、 Insulin-like growth factor 、 Intracellular 、 Molecular biology 、 Receptor 、 Transfection 、 Cell growth 、 Insulin-Like Growth Factor Receptor
摘要: The type 1 insulin-like growth factor receptor (IGF-IR) is emerging as a powerful survival against variety of apoptotic agents in many cell types. A mutant IGF-IR designated 486/STOP known to induce apoptosis and inhibit the human tumor cells mice. We have investigated mechanism action 486/STOP. To study it, we developed new retroviral vector which combined self-inactivating 5′-long terminal repeat with an inducible heat-shock promoter (heat shock protein 70) from Drosophila . Using this technique, find that polypeptide encoded by partially retained within secreted. However, secreted subsequently taken up cells. In both cases, specific intracellular interaction endogenous IGF-IRs can be demonstrated coimmunoprecipitation.
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