Monoclonal antibody 7D5 raised to cytochrome b558 of human neutrophils: immunocytochemical detection of the antigen in peripheral phagocytes of normal subjects, patients with chronic granulomatous disease, and their carrier mothers
作者： M Nakamura , M Murakami , T Koga , Y Tanaka , S Minakami
DOI: 10.1182/BLOOD.V69.5.1404.1404
关键词: Phagocyte 、 Immunology 、 Antigen 、 Monoclonal antibody 、 Chronic granulomatous disease 、 Biology 、 Cytochrome b 、 Cytochrome 、 Immunocytochemistry 、 Antibody 、 Molecular biology
摘要: We have established a monoclonal hybridoma clone that produces IgG1 against the cytochrome b558 of human neutrophils. The antibody 7D5, secreted by hybridoma, bound to solubilized b neutrophils but not other proteins such as hemoglobin, myeloperoxidase, and pig P-450. Immunocytochemical studies normal peripheral blood showed 7D5 monocytes lymphocytes or erythrocytes. male patients female patient with chronic granulomatous disease lacked antigen well absorption spectrum for b558. A mosaic antigen-positive -negative was observed in mothers patients. These biochemical immunocytochemical results indicate is specific phagocytes.
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