Genome-Wide Survey and Analysis of Microsatellite Sequences in Bovid Species

作者: Wen-Hua Qi , Xue-Mei Jiang , Lian-Ming Du , Guo-Sheng Xiao , Ting-Zhang Hu

DOI: 10.1371/JOURNAL.PONE.0133667

关键词: Capra hircusGenome sizeBubalusBiologyGeneticsComparative genomicsGenomeRelative species abundanceMicrosatelliteGenetic markerGeneral Biochemistry, Genetics and Molecular BiologyGeneral Agricultural and Biological SciencesGeneral Medicine

摘要: Microsatellites or simple sequence repeats (SSRs) have become the most popular source of genetic markers, which are ubiquitously distributed in many eukaryotic and prokaryotic genomes. This is first study examining comparing SSRs completely sequenced genomes Bovidae. We analyzed compared number SSRs, relative abundance, density, guanine-cytosine (GC) content proportion six taxonomically different bovid species: Bos taurus, Bubalus bubalis, mutus, Ovis aries, Capra hircus, Pantholops hodgsonii. Our analysis revealed that, based on our search criteria, total perfect found ranged from 663,079 to 806,907 covered 0.44% 0.48% Relative abundance density these Bovinae were non-significantly correlated with genome size (Pearson, r 0.05). Perfect mononucleotide abundant, followed by pattern: di- > tri- penta- tetra- hexanucleotide SSRs. Generally, decreased as motif repeat length increased each species The GC-content was trinucleotide least GC-contents pentanucleotide showed a great deal similarity among chromosomes B. O. C. hircus. SSR all O.aries, hircus closely positively chromosome 0.980, p abundances same nucleotide type

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