Identification and characterization of short tandem repeats in the Tibetan macaque genome based on resequencing data.

摘要: The Tibetan macaque, which is endemic to China, currently listed as a Near Endangered primate species by the International Union for Conservation of Nature (IUCN). Short tandem repeats (STRs) refer repetitive elements genome sequence that range in length from 1-6 bp. They are found many organisms and widely applied population genetic studies. To clarify distribution characteristics genome-wide STRs understand their variation among macaques, we conducted survey with next-generation sequencing five macaque samples. A total 1 077 790 perfect were mined our assembly, an N50 4 966 Mono-nucleotide most abundant, followed tetra- di-nucleotide repeats. Analysis GC content showed consistent results other macaques. Furthermore, using STR analysis software (lobSTR), proportion base pair deletions was greater than insertions individuals (P<0.05, t-test). We also number homozygous heterozygous t-test), Emei Jianyang macaques showing more loci Huangshan mean alleles slightly higher those individuals, thus revealing differences allele size between two populations. polymorphic identified based on reference good amplification efficiency could be used study genetics neighbor-joining tree classified into different branches according geographical origin, indicating high differentiation Sichuan elucidated provided effective method screening STRs. Our lay foundation future studies