Pig BMSCs Transfected with Human TFPI Combat Species Incompatibility and Regulate the Human TF Pathway in Vitro and in a Rodent Model

摘要: Background: The activation of tissue factor (TF) is one the major reasons for coagulation dysregulation after pig-to-primate xenotransplantation. Tissue pathway inhibitor (TFPI) most important TF. Studies have demonstrated species incompatibility between pig TFPI and human Methods: A pig-to-macaque heterotopic auxiliary liver transplantation model was established to determine origin activated Chimeric proteins were constructed assess role Kunitz domains in incompatibility. Immortalised bone marrow mesenchymal stem cells transfected with tested their ability inhibit clotting vitro. Results: TF from recipient early Pig domain 2 bound FXa, but 1 did not effectively TF/FVIIa. (BMSCs) showed a prolonged recalcification time vitro rodent model. Conclusion: Recipient relevant dysregulated plays TF, can be inhibited by TFPI-transfected BMSCs. Our study shows possible way resolve TFPI.