Liver NK1.1+ CD4+ alpha beta T cells activated by IL-12 as a major effector in inhibition of experimental tumor metastasis.

摘要: We demonstrate herein evidence that IL-12-activated alpha beta T cells with intermediate TCR (NK1+ TCRint cells) in the liver inhibit metastases lung as well of i-v. injected tumors. IL-12 administration enhanced NK1 expression NK1+ (NK1high) and increased CD4 weakly positive (CD4low) cells, while both CD4+ double-negative were proportionally diminished. Accordingly, major parts NK1high are CD4low most these V 8+ cells. The cytotoxic assays IL-12-stimulated hepatic mononuclear after treatment respective Abs complement vitro sorting revealed effectors. When (but not splenocytes) transferred into tumor-preinjected mice, EL-4 cell 3LL inhibited. antimetastasis transfer was abrogated by depletion CD3+ or but CD8+ before transfer. Moreover, nude mice also inhibited organs. Although nearly absent vein blood, a significant proportion appeared administration. These results including extrathymic ones, effectors against tumor metastasis suggest migrate metastases.