Electrophoretic Heterogeneity of α-Glycerophosphate Dehydrogenase among many Species of Drosophila

摘要: Coyne, J. A., W. F. Eanes, A. M. Ramshaw (Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts 02138) and R. K. Koehn (Department Ecology Evolution, State University New York, Stony Brook, York 11794). 1979. Electrophoretic heterogeneity o-glycerophosphate dehydrogenase among many species Drosophila. Syst. Zool. 28:164-175.-Electromorphic variation a-glycerophosphate in 71 Drosophila has been studied using sequential polyacrylamide gel electrophoresis, starch isoelectric focusing. Although this locus was previously reported as nearly invariant the genus, study shows a substantial increase between which is concordant with known taxonomy genus. However, these methods revealed no additional within themselves. Each three found not detectable by other two; thus, all are necessary for maximizing observed species. In comparison to previous electrophoretic studies enzyme Drosophila, new data show much increased concordance immunological kinetic differences different The implications results speciation discussed. [Drosophila spp.; dehydrogenase; electrophoresis; heterogeneity.] recent years electrophoresis extensively used investigate questions general interest evolutionary biology systematics (Ayala et al., 1974; King Wilson, 1975; Mickevich Johnson, 1976; others). fairly simple gather, it number advantages over more traditionally taxonomic characters (Avise, 1974). Because assumed that electromorphs only step removed from single genes, them readily translated into measures "genetic distance." Inherent investigations assumptions about nature variation. It often recognized some genetic may be mistakenly described identities due convergence variants similar mobilities; but problem usually ignored, being considered insignificant given weight total set. Of course generalities drawn such can seriously depend on safety assumptions. Evidence now accumulating phenotypes necessarily homogeneous molecular entities, heterogeneous classes will divide when subjected series conditions (e.g., Weitkamp 1973; Bernstein Singh Coyne Felton, 1977). When species, approach had significant effect interpretation particular problems, descriptions geographic gene frequencies. So far analyses have extended comparisons undetected at level even profound than report we examine apparent identity (a-GPDH, Lglycerol-3-phosphate oxidoreductase, E.C. 1.1.1.8.) genus Our clearly demonstrates judgements