Identification of reference genes for gene expression normalization in safflower (Carthamus tinctorius)

摘要: Safflower (Carthamus tinctorius L., Asteraceae) is an important oil crop and medicinal plant. Gene expression analysis gaining importance in the research of safflower. Quantitative PCR has become a powerful method for gene study. Reference genes are one major qualification requirements qPCR because they can reduce variability. To identify reference safflower, nine candidate housekeeping were selected from EST library safflower constructed by our lab: CtACT (actin), CtGAPDH (glyceraldehyde 3-phosphate dehydrogenase), CtE1F4A (elongation factor 1 alpha), CtTUA (alpha-tubulin), CtTUB (beta-tubulin), CtPP2A (serine/threonine-protein phosphatase), (eukaryotic initiation 4A), CtUBI (Ubiquitin), Ct60S (60S acidic ribosomal protein). Expression stability was examined across 54 samples, representing tissues at different flowering stages two chemotype lines. We assessed these employing four algorithms (geNorm, NormFinder, ΔCt approach, BestKeeper) found that highly ranked genes. used as to evaluate CtFAD2-10 CtKASII. Our data suggest could be internal controls normalize