Pluronics-Formulated Farnesol Promotes Efficient Killing and Demonstrates Novel Interactions with Streptococcus mutans Biofilms.

摘要: Streptococcus mutans is the primary causative agent of dental caries, one most prevalent diseases in United States. Previously published studies have shown that Pluronic-based tooth-binding micelles carrying hydrophobic antimicrobials are extremely effective at inhibiting S. biofilm growth on hydroxyapatite (HA). Interestingly, these also demonstrated non-binding (NBM) antimicrobial had an inhibitory effect, leading to hypothesis Pluronic themselves may interact with biofilm. To explore this potential interaction, three different strains were each grown as tissue culture plates, either untreated or supplemented NBM alone (P85), containing farnesol (P85F), (F). In tested strain, biomass was significantly decreased (SNK test, p < 0.05) P85F and F biofilms relative biofilms. Furthermore, formed large towers dead cells not observed other treatment conditions. Tower formation appeared be specific formulated farnesol, phenomenon triclosan. Parallel CFU/ml determinations revealed presence resulted a 3-log reduction viability, whereas viability by less than 1-log. Wild-type absence sucrose gtfBC mutant did form towers. However, increased cell killing still observed, suggesting independent tower formation. Finally, repeated pre-formed able elicit 2-log parallel only reduced 0.5-log. Collectively, results suggest Pluronics-formulated induces alterations architecture, presumably via interaction sucrose-dependent matrix, viable option prevention pathogenic plaque