Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
作者： Jinhua Liu , Yanyu Shi , Siying Teng , Lianpeng Wu , Xinmin Zhang
DOI: 10.1155/2017/9319035
关键词: Routine analysis 、 Food science 、 Human health 、 TaqMan 、 Animal science 、 Nyctereutes procyonoides 、 Dna concentration 、 Detection limit 、 DNA extraction 、 Loop-mediated isothermal amplification 、 Biology
摘要: Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat injurious to human health. Currently, no rapid and sensitive method detecting raccoon in mixtures available. In this study, we developed easily applicable, rapid, feasible identifying the presence of based on loop-mediated isothermal amplification (LAMP). Four sets LAMP primers were tested at different temperatures, that worked best 62°C (set 2) determined. assay, there was cross-reactivity with procured from other species animals detection limit DNA concentration 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), sensitivity 0.1 pg·μL−1 complies most requirements routine analysis. Moreover, by method, containing more 0.5% component directly detected (without extraction) supernatant isolated after performing repeated cycles thawing freezing minced mixtures. Our results show assay a valuable, straightforward, tool identification
hindawi.com
core.ac.uk
cabdirect.org参考文章(31)
Martin C. W. Chan, Paul K. S. Chan, Tracing the SARS-coronavirus Journal of Thoracic Disease. ,vol. 5, ,(2013) , 10.3978/J.ISSN.2072-1439.2013.06.19
CN, Jr Stewart, Laura E Via, A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques. ,vol. 14, pp. 748- 750 ,(1993)
Guoshun Chen, Yanan Sui, Sheng Chen, Detection of flavor compounds in longissimus muscle from four hybrid pig breeds of Sus scrofa, Bamei pig, and Large White Bioscience, Biotechnology, and Biochemistry. ,vol. 78, pp. 1910- 1916 ,(2014) , 10.1080/09168451.2014.936348
Wolfgang Frank, Emil C. Reisinger, Wiltrud Brandt-Hamerla, Ilona Schwede, Werner Handrick, Mycobacterium microti--pulmonary tuberculosis in an immunocompetent patient. Wiener Klinische Wochenschrift. ,vol. 121, pp. 282- 286 ,(2009) , 10.1007/S00508-009-1164-0
Barbara Druml, Walter Mayer, Margit Cichna-Markl, Rupert Hochegger, Development and validation of a TaqMan real-time PCR assay for the identification and quantification of roe deer (Capreolus capreolus) in food to detect food adulteration Food Chemistry. ,vol. 178, pp. 319- 326 ,(2015) , 10.1016/J.FOODCHEM.2015.01.003
R. Kubota, B. G. Vine, A. M. Alvarez, D. M. Jenkins, Detection of Ralstonia solanacearum by Loop-Mediated Isothermal Amplification Phytopathology. ,vol. 98, pp. 1045- 1051 ,(2008) , 10.1094/PHYTO-98-9-1045
Wenhui Nie, Jinhuan Wang, Polina Perelman, Alexander S. Graphodatsky, Fengtang Yang, Comparative chromosome painting defines the karyotypic relationships among the domestic dog, Chinese raccoon dog and Japanese raccoon dog. Chromosome Research. ,vol. 11, pp. 735- 740 ,(2003) , 10.1023/B:CHRO.0000005760.03266.29
Bo Hou, Xianrong Meng, Liyuan Zhang, Jinyue Guo, Shaowen Li, Hui Jin, None, Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken, duck and goose DNA in meat products Meat Science. ,vol. 101, pp. 90- 94 ,(2015) , 10.1016/J.MEATSCI.2014.11.007
Antonio M. Gomez Galan, Marcel Brohée, Eugénia de Andrade Silva, Arjon J. van Hengel, Hubert Chassaigne, Development of a real-time PCR method for the simultaneous detection of soya and lupin mitochondrial DNA as markers for the presence of allergens in processed food Food Chemistry. ,vol. 127, pp. 834- 841 ,(2011) , 10.1016/J.FOODCHEM.2011.01.019
A Macedo-Silva, S.F.C Barbosa, M.G.A Alkmin, A.J Vaz, M Shimokomaki, A Tenuta-Filho, Hamburger meat identification by dot-ELISA Meat Science. ,vol. 56, pp. 189- 192 ,(2000) , 10.1016/S0309-1740(00)00039-5