SP1‐mediated lncRNA PVT1 modulates the proliferation and apoptosis of lens epithelial cells in diabetic cataract via miR‐214‐3p/MMP2 axis
作者： Jun Yang , Shaozhen Zhao , Fang Tian
DOI: 10.1111/JCMM.14762
关键词: Sp1 transcription factor 、 Transcription (biology) 、 Pathogenesis 、 PVT1 、 miR-214 、 MMP2 、 Chemistry 、 Apoptosis 、 Promoter 、 Cell biology
摘要: Emerging evidence illustrates the critical roles of long non-coding RNAs (lncRNAs) in diabetes. However, deepgoing regulation lncRNA PVT1 diabetic cataract (DC) is still unclear. Here, present research investigates pathologic and underlying mechanism by which regulates DC pathogenesis. Human lens epithelial (HLE) B-3 cells were induced high glucose (HG) to simulate microenvironment models. Results revealed that expression was up-regulated HG-induced HLE as compared normal group. Transcription factor SP1 could bind with promoter region activate its transcription. Functionally, knock-down repress proliferation promote apoptosis cells. Mechanistically, acted 'miRNA sponge' target miR-214-3p/MMP2 axis. This finding a novel insight for pathogenesis, providing an inspiration mechanism.
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