Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo.

摘要: Matrix metalloproteinases (MMPs) constitute a large group of endoproteases that play important functions during embryonic development, tumor metastasis and angiogenesis by degrading components the extracellular matrix. Within this family, we focused our study on Mt4-mmp (also called Mmp17) belongs to distinct subset is anchored cell surface via glycosylphosphatidylinositol (GPI) moiety with catalytic site exposed space. Information about its function substrates very limited date, little has been reported role in developing embryo. Here, report detailed expression analysis mouse development using LacZ reporter transgenic line. We showed detected from early stages postnatal following dynamic restricted pattern expression. was first at E8.5 intersomitic vascularization, endocardial endothelium dorsal aorta. Mt4-mmpLacZ/+ cells were also observed neural crest cells, somites, floor plate notochord stages. From E10.5, localized limb buds persists development. A strong brain begins E12.5 continues Specifically, staining olfactory bulb, cerebral cortex, hippocampus, striatum, septum, thalamus spinal cord. In addition, LacZ-positive eye initially hyaloid artery later located lens retina. confirmed quantitative RT-PCR western blot some tissues. Our data point for metalloproteinase particularly formation,