Identification and distribution of three serologically undetected alleles of HLA-DR by oligonucleotide.DNA typing analysis.

摘要: Abstract Recent progress in the molecular biology of human major histocompatibility complex class II genes (HLA-DP, -DQ, -DR) have shown that genetic complexity and allelic polymorphism are greater than expected. In case HLA-DR, three DR beta-chain loci been identified linked, two which (DR beta I III, now assigned names HLA-DRIB HLA-DR3B) functional. We HLA micropolymorphism detected at DNA sequence level can easily be analyzed by hybridization with allele-specific oligonucleotides (HLA "oligotyping"). DRw52 supertypic specificity, includes DR3, DR5, DRw6, DRw8 haplotypes, alleles, referred to as DRw52a, DRw52b, DRw52c, recently HLA-DR3B locus sequencing. Hybridization locus- oligonucleotide probes (designated 52a, 52b, 52c) has performed on from normal individuals forming a panel 82 haplotypes establish distribution these alleles. Individuals DR3 haplotype had either DRw52a or DRw52b allele, extended HLA-A1,B8,DR3 only allele. DR5 all while DRw6 -52b, -52c None alelles found individuals. Analysis this micropolymorphism, undetectable common typing procedures, is therefore operational for more accurate matching transplantation improving correlations between disease susceptibility.