Detection of a novel DNA virus (TTV) sequence in peripheral blood mononuclear cells.
作者： Akiko Okamura , Mikio Yoshioka , Mitsuru Kubota , Hideaki Kikuta , Hiroaki Ishiko
DOI: 10.1002/(SICI)1096-9071(199906)58:2<174::AID-JMV12>3.0.CO;2-X
关键词: DNA virus 、 Population 、 Cord blood 、 Virology 、 Biology 、 DNA sequencing 、 Peripheral blood mononuclear cell 、 Polymerase chain reaction 、 DNA 、 Virus
摘要: DNA sequences of a novel virus (TTV) were examined in 81 peripheral blood mononuclear cell (PBMC) samples from 48 children and 33 adults, 22 cord cells (CBMC) samples, 7 autopsy liver tissue by hemi-nested polymerase chain reaction (PCR). The PCR was carried out using the published primers (NG059, NG061, NG063) to amplify TTV sequences. detected 4 (5%) PBMC none (0%) CBMC 2 (29%) direct gel analysis. PCR-amplified products confirmed sequencing. sequencing showed considerable diversities, with differences 0-55% 6 isolates, compared prototype sequence TTV. These results suggest that is ubiquitous produces asymptomatic infection large proportion general population without transfusion blood-derived products. To our knowledge, this first report describing detection PBMCs.
sci-hub.se 参考文章(5)
Tsutomu Nishizawa, Hiroaki Okamoto, Keiko Konishi, Hiroshi Yoshizawa, Yuzo Miyakawa, Makoto Mayumi, A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochemical and Biophysical Research Communications. ,vol. 241, pp. 92- 97 ,(1997) , 10.1006/BBRC.1997.7765
Hideaki Kikuta, Yuichi Taguchi, Kazuhiro Tomizawa, Kimikazu Kojima, Nobuaki Kawamura, Akihito Ishizaka, Yukio Sakiyama, Shuzo Matsumoto, Shosuke Imai, Toshifumi Kinoshita, Shigeki Koizumi, Toyoro Osato, Ichiro Kobayashi, Isamu Hamada, Kanji Hirai, Epstein-Barr virus genome-positive T lymphocytes in a boy with chronic active EBV infection associated with Kawasaki-like disease Nature. ,vol. 333, pp. 455- 457 ,(1988) , 10.1038/333455A0
ED Heegaard, A Hornsleth, Parvovirus: the expanding spectrum of disease Acta Paediatrica. ,vol. 84, pp. 109- 117 ,(1995) , 10.1111/J.1651-2227.1995.TB13591.X
Hiroaki Okamoto, Tsutomu Nishizawa, Naomi Kato, Masato Ukita, Hiroki Ikeda, Hisao Iizuka, Yuzo Miyakawa, Makoto Mayumi, Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology Hepatology Research. ,vol. 10, pp. 1- 16 ,(1998) , 10.1016/S1386-6346(97)00123-X
Kenneth I. Berns, Roy A. Bohenzky, Adeno-associated viruses: an update. Advances in Virus Research. ,vol. 32, pp. 243- 306 ,(1987) , 10.1016/S0065-3527(08)60479-0