Studies on anti-hepatoma activity of Annona squamosa L. pericarp extract.
作者： Ya-yun Chen , Yu-zhu Cao , Fu-qiang Li , Xiao-li Zhu , Chen-xiao Peng
DOI: 10.1016/J.BMCL.2017.03.036
关键词: Annona squamosa 、 Ethyl acetate 、 Chemistry 、 Phytochemistry 、 Terpenoid 、 MTT assay 、 Biochemistry 、 Phytochemical 、 In vitro 、 Flow cytometry
摘要: This study investigated the anti-hepatoma activity of different extracts from A. squamosa pericarps, phytochemistry ethyl acetate (EtOAc) fraction and possible mechanism active constituents. The pericarps were evaluated by MTT assay against SMMC-7721 cells in vitro verified using H22 xenografts bearing mice. Phytochemical investigation pericarp extract was carried out. pro-apoptosis cycle arrest effects constituents observed fluorescent microscope flow cytometry. Western blot conducted to find mechanisms result showed that EtOAc fraction. Two ent-kaurane diterpenoids, named ent-kauran-16-en-19-oic acid ent-kauran-15-en-19-oic acid, isolated G1 phase these diterpenoids found. could activate caspase-3,-8,-9, up-regulate Bax down-regulate Bcl-2.
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