Elution of Exocellular Enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae
作者： Ralph Weimberg , William L. Orton
DOI: 10.1128/JB.91.1.1-13.1966
关键词: Biology 、 Microbiology 、 Saccharomyces 、 Glycoside hydrolase 、 Biochemistry 、 Acid phosphatase 、 Saccharomyces cerevisiae 、 Digestion 、 Invertase 、 Enzyme 、 Elution 、 Molecular biology
摘要: Weimberg, Ralph (Northern Regional Research Laboratory, Peoria, Ill.), and William L. Orton. Elution of exocellular enzymes from Saccharomyces fragilis cerevisiae. J. Bacteriol. 91:1-13. 1966.-Invertase acid phosphatase are repressible in S. The conditions for eluting these both organisms were compared. Either KCl or beta-mercaptoethanol eluted the fragilis, amounts varied quantitatively according to physiological age organism. In addition enzymatic activity cells, reagents also caused a large increase amount that remained associated with cells fragilis. Invertase not removed cerevisiae by beta-mercaptoethanol. These separated only when there was some degree cell-wall digestion snail gut fluid.
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