Increased expression of gelatinases and collagenase in rat lungs exposed to 100% oxygen
作者： A Pardo , M Selman , K Ridge , R Barrios , J I Sznajder
DOI: 10.1164/AJRCCM.154.4.8887609
关键词: Lung injury 、 Gelatinase 、 Pathology 、 Lung 、 Bronchoalveolar lavage 、 Gelatinases 、 Matrix metalloproteinase 、 Biology 、 Interstitial collagenase 、 Molecular biology 、 Collagenase
摘要: Exposure of adult rats to 100% O2 produces a lethal injury by 72 h. We reasoned that matrix metalloproteinases participate in the pathogenesis hyperoxic lung injury. To end we studied expression and activity gelatinases A B interstitial collagenase tissues bronchoalveolar lavage fluids (BALF) exposed oxygen for 60 Gelatin zymography BALF samples revealed 472 kDa molecular species both controls oxygen-exposed animals. In addition, from exhibited 95-kDa gelatinase. Likewise, total gelatinolytic collagenolytic activities were significantly increased rats. situ hybridization an increase type IV collagenases as well mRNAs lungs. The three enzymes expressed alveolar macrophages, variable degrees epithelial cells. Immunoreactive gelatinase paralleled cell localization of...
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