Xist lncRNA forms silencing granules that induce heterochromatin formation and repressive complexes recruitment by phase separation

摘要: Xist long non-coding RNA induces heterochromatinization of the X-chromosome by accumulating in large ribonucleoprotein assemblies. We suggest that regulates X chromosome inactivation recruiting RNA-binding proteins via a mechanism liquid-liquid phase separation. To support this hypothesis, we provide evidence forms condensates are similiar size, shape and protein composition to paraspeackles stress granules. show interactome is particularly enriched intrinsically disordered capable separating. reinforces Xi sequestering repressive complexes, such as Polycomb proteins, an indirect way through This process complementary canonical chromatin-mediated recruitment.