FHIT protein expression and its relation to apoptosis, tumor histologic grade and prognosis in colorectal adenocarcinoma: an immunohistochemical and image analysis study.

摘要: The FHIT gene, a member of the histidine triad family has been identified as tumor suppressor gene. Molecular genetic approaches to determine alterations in gene colorectal cancers have produced varying results with reported abnormalities transcripts 13% 50% cases studied. be involved regulation apoptosis and cell cycle culture systems. Immunohistochemical (IHC) studies expression human colon cancer its correlation clinical prognosis sparse. We studied 100 by IHC computerized image analysis (CIA) method evaluate rate tumors corresponding mucosae. Follow-up data for at least five years was available all patients. correlated grade, stage prognosis. used commercially polyclonal anti antibody Apoptaq kit on paraffin-embedded their mucosae SAMBA 4000 CIA system labeling index (LI), mean optical density (MOD) stain calculate quick score (QS). LI is ratio positively stained areas total area tissues examined, MOD represents concentration positive measured per pixels QS numeric product each microscopic examined. Image staining sections defined three main groups based reactivity antibody. Group I included 23 where less than 55% 36%. Eight this group showed complete loss expression. II 41 between 65% 60%. III composed 36 more 69%. Our that absence or reduction protein tumors, relative morphologically normal mucosa, plays role development few (23%). Poorly differentiated carcinomas absent decreased FHIT. A distant metastases worse Over-expression directly proportional apoptotic provides an objective accurate assessment patterns generates numerical evaluating intensity better depending subjective light microscopy alone.