Quantitative TLC Analysis of Sterol (24β-Ethylcholesta-5,22E,25-triene-3β-ol) in Agnimantha (Clerodendrum phlomidis Linn)
作者： Karuna Shanker , Subhash Chandra Singh , Sangita Pant , Pooja Srivastava , Akhilesh Kumar Yadav
DOI: 10.1365/S10337-007-0502-5
关键词: Chemistry 、 Derivatization 、 Silica gel 、 Quantitative analysis (chemistry) 、 Clerodendrum phlomidis 、 Reagent 、 Thin-layer chromatography 、 High performance thin layer chromatography 、 Detection limit 、 Chromatography
摘要: A quantitative method using silica gel 60F254 high performance thin layer chromatography plates, automated bandwise sample application, and visible mode densitometric has been developed for the determination of 24b-ethylcholesta-5,22E,25-triene-3b-ol (ECTO) in aerial part Clerodendrum phlomidis. ECTO was used as a chemical marker standardization C. phlomidis plant extracts. The separation performed on TLC plates chloroform-methanol (98.5: 1.5, v/v) mobile phase. quantitation carried out reflection/absorption at 650 nm after post chromatographic derivatization with anisaldehyde reagent. precise accurate quantification can be linear working concentration range 150‐ 400 ng band 1 good correlation (r 2 = 0.996). validated peak purities, precision, robustness, limit detection (LOD) (LOQ), etc. per ICH guidelines.
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