Identification of transmembrane proteins as potential prognostic markers and therapeutic targets in breast cancer by a screen for signal sequence encoding transcripts

摘要: Identification of transmembrane proteins as potential prognostic markers and therapeutic targets in breast cancer by a screen for signal sequence encoding transcripts This study demonstrates, through combination stringent screening methods thorough validation, that it is possible to identify preferentially expressed primary tumour cells. mRNA was extracted from cells isolated invasive cancers then subtracted against normal tissue prior the generation sequence-trap library. Screening library identified 31 positive clones 12 cell-surface secreted proteins. The expression subset genes interrogated using high-throughput method (tissue microarray) coupled with cutting-edge situ techniques large cohort patients who had undergone uniform adjuvant chemotherapy. Expression CD98 heavy chain (CD98HC) low-level insulin-like growth factor 2 receptor/mannose-6-phosphate receptor (IGF2R1M6PR) correlated poor patient prognosis whole cohort. bradykinin B1 (BDKRB1) testis enhanced gene transcript (TEGT) good woman oestrogen (ER)-negative tumours. These results indicate this combined approach isolating cells, generating specifically isolate signal-sequence-containing transcripts, hybridization on microarrays successfully novel (BDKRB1, CD98hc, TEGT) (CD98hc) cancer. Copyright (c) 2006 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.