Temporal relationship of free radical-induced lipid peroxidation and loss of latent enzyme activity in highly enriched hepatic lysosomes.

摘要: Loss of latency due to membrane lipid peroxidation induced in vitro was studied highly purified rat liver lysosomes. Enriched fractions lysosomes were isolated by free flow electrophoresis. Lipid lysosomes, assayed as malondialdehyde formation, catalyzed a radical generating system consisting dihydroxyfumaric acid and Fe3+-ADP. The reaction occurred readily at 37 degrees C reached plateau 10 min; however, the loss lysosomal latency, determined increased percentage beta-N-acetylglucosaminidase activity, more gradually maximum after 30 min. Scavengers superoxide, hydrogen peroxide, singlet oxygen, hydroxyl radicals did not inhibit nor prevent latency. However, preincubation with alpha-tocopherol effectively blocked induction substantially reduced These results indicate that is susceptible radical-induced peroxidation; further, this process may be immediate cause subsequent disintegration lysosome. nature protective effect unclear but its interaction unsaturated lipids interruption chain-reaction initiated radicals.