Combined use of anticancer drugs and an inhibitor of multiple drug resistance-associated protein-1 increases sensitivity and decreases survival of glioblastoma multiforme cells in vitro.

摘要: Glioblastoma multiforme (GBM) is a brain tumour characterised by remarkably high chemoresistance and infiltrating capability. To date, chemotherapy with temozolomide has contributed only poorly to improved survival rates in patients. One of the most important mechanisms comes about through activity certain proteins from ATP-binding cassette superfamily that extrudes antitumour drugs, or their metabolites, cells. We identify an increased expression multiple drug resistance-associated protein 1 (Mrp1) glioblastoma biopsies T98G G44 cell lines. The this transporter was also confirmed measuring extrusion fluorescent substrate CFDA. sensitivity GBM cells low upon exposure temozolomide, vincristine etoposide, decreases viability below 20% seen at therapeutic concentrations these drugs. However, combined etoposide inhibitor Mrp1 efficiently decreased up 80%. conclude chemosensitization inhibitors might be efficient tool for treatment human GBM.