Method for detecting residual quantity of 18 sulfanilamide drugs in beef and mutton

摘要: The invention discloses a method for detecting residual quantity of 18 sulfanilamide drugs in beef and mutton. comprises taking 5g uniform mutton, adding anhydrous magnesium sulfate, sodium acetate acetonitrile solutions into the carrying out high-speed homogenized extraction, centrifugation, vortex mixing purification impurity-removal on supernatant by an adsorbent containing plurality materials, pressure reduction or nitrogen blowing condensation subjected to re-centrifugation until drying, redissolving residues, after shaking-up, filtering solution microfiltration membrane with pore sizes 0.22 micrometers obtain sample solution, separation reversed phase column filled C18 as filler, ultraviolet light on-line rapid derivation, detection fluorescence detector, qualitative analysis according chromatographic peak retention time, quantification external standard calculating has simple processes, utilizes less amount reagent, high sensitivity, releases simultaneous one step, greatly shortens time good application prospect.