Vasohibin 2 promotes human luminal breast cancer angiogenesis in a non-paracrine manner via transcriptional activation of fibroblast growth factor 2

摘要: Vasohibin 2 (VASH2) is an angiogenic factor and cancer-related protein that acts via paracrine mechanisms. Here, we investigated the function mechanism of action VASH2 in 200 human breast cancer tissues by performing immunohistochemical staining, western blot, indirect sandwich enzyme-linked immunosorbent assay (ELISA), a semi-quantitative sandwich-based antibody array. Breast cells stably overexpressing or with knocked-down were established used for in vivo in vitro models. In luminal tissue, but not HER2-positive basal-like tissues, was positively correlated CD31-positive microvascular density, induced angiogenesis xenograft tumors, promoted umbilical vein endothelial cell tube formation in vitro. expression absent concentrated conditioned medium collected from VASH2-overexpressing cells. Further, regulated fibroblast growth (FGF2) cells, pro-angiogenic effect overexpression blocked FGF2 neutralization Additionally, dual luciferase reporter Chromatin immunoprecipitation analysis results showed promoter transcriptionally activated histone modifications. conclusion, promotes transcriptional activation through non-paracrine