Testicular embryonal carcinoma cell proliferation in vitro: Effect of B152, an anti-hyperglycosylated hCG monoclonal antibody

摘要: Hyperglycosylated human chorionic gonadotropin (hCG-H), a variant of hCG, has been shown to promote the growth and invasion trophoblast cells in early pregnancy placental implantation, choriocarcinoma testicular germ cell malignancies. In previous study (15), hCG-H was found have significant effect on line, JEG3, embroyonal carcinoma NTERA. It also that, B152, an anti-hCG-H specific monoclonal antibody, significantly inhibited these vitro vivo murine models. this study, we quantified amount produced by two embryonal lines, n2102Ep NTERA 2.clone D1 (NT2/D1). We then investigated impact B-152 proliferation each line as well JEG-3. Over 72 hour period, produce 0.04 mIU hCG per 10,000 0.004 cells. The proportion 10.0%. NT2/D1 0.02 0.002 11.5%. cultured lines JEG-3 seven different concentrations B152 ranging from 0.0 μg/mL 40 for hours. Cell determined measuring absorbance determining percentage relative control without additional B152. decreased with increasing all three significance Bartholomew’s Test means: (P value <0.001), 0.005), 0.001). Results suggest that had negative n2102Ep, NT2/D1, conclude hyperglycosylated appears inhibit