Genetic structure of Plasmodium falciparum field isolates in eastern and north-eastern India

摘要: Molecular techniques have facilitated the studies on genetic diversity of Plasmodium species particularly from field isolates collected directly patients. The msp-1 and msp-2 are highly polymorphic markers large allelic polymorphism has been reported in block 2 gene central repetitive domain (block3) gene. Families differing nucleotide sequences number (length variation) were used for genotyping purposes. As limited reports available existing among falciparum population India, this report evaluates extent P. eastern north-eastern regions India. A study was designed to assess India using allele specific nested PCR assays sequence analysis. Field five sites distributed three states namely, Assam, West Bengal Orissa. diverse respect length as well motifs with prevalence all families msp-2. Prevalence identical composition high level identity alleles suggest a considerable amount flow between populations different states. comparatively higher proportion multiclonal multiplicity infection (MOI) observed malarious districts Karbi Anglong (Assam) Sundergarh (Orissa). In sites, R033 family be monomorphic an size 150/160 bp. 80–90% Indian data other suggests that is mixture strains. present shows (block 2) (central repeat region, 3). expected picture closer southeast Asia, Papua New Guinea Latin American countries, low meso-endemicity malaria comparison African hyper- holo-endemicity.