Detection of ras point mutations by polymerase chain reaction using mutation-specific, inosine-containing oligonucleotide primers
作者： Thomas Ehlen , Louis Dubeau
DOI: 10.1016/0006-291X(89)92452-2
关键词: Biology 、 Polymerase chain reaction 、 Inverse polymerase chain reaction 、 Primer (molecular biology) 、 Multiplex polymerase chain reaction 、 COLD-PCR 、 Oligonucleotide 、 Mutation 、 Point mutation 、 Molecular biology
摘要: Abstract The use of DNA primers with 3′-ends complementary to specific genetic point mutations allowed for the rapid detection such in genomic by polymerase chain reaction. sensitivity this approach was that could be detected samples mixed a 10 7 -fold excess normal non-mutated DNA. To increase practicality affecting all 3 known ras oncogenes we synthesized mutation-specific genes substituting inosine residues at positions corresponding ambigous bases on genes.
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