The histone variant H2A.Z is an important regulator of enhancer activity.

摘要: Gene regulatory programs in different cell types are largely defined through cell-specific enhancers activity. The histone variant H2A.Z has been shown to play important roles transcription mainly by controlling proximal promoters, but its effect on enhancer functions remains unclear. Here, we demonstrate genome-wide approaches that is present at a subset of active bound the estrogen receptor alpha (ERα). We also determine does not influence local nucleosome positioning around ERα using ChIP sequencing nucleosomal resolution and unsupervised pattern discovery. further highlight H2A.Z-enriched associated with chromatin accessibility, H3K122ac enrichment hypomethylated DNA. Moreover, upon stimulation, occupied produce RNAs (eRNAs), recruit RNA polymerase II as well RAD21, member cohesin complex involved interactions between promoters. Importantly, their recruitment eRNAs production abolished depletion, thereby revealing novel functional link occupancy Taken together, our findings suggest acts an player for establishing maintaining environment required recruitment, enhancer-promoters interactions, all essential attributes