Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani.
作者： Aarthi Venugopalan , Uma Rani Potunuru , Madhulika Dixit , Smita Srivastava
DOI: 10.1016/J.BIORTECH.2016.01.079
关键词: Elicitor 、 Blood serum 、 Fermentation 、 Bovine serum albumin 、 Biochemistry 、 Food science 、 Endophyte 、 Camptothecin 、 Biology 、 Tryptamine 、 Fusarium solani
摘要: Volumetric productivity of camptothecin from the suspension culture endophyte Fusarium solani was enhanced up to ∼152 fold (from 0.19 μg l(-1) d(-1) 28.9 d(-1)) under optimized fermentation conditions including initial pH (6.0), temperature (32 °C) and agitation speed (80 rpm) with (5% (v/v)) ethanol as medium component. Among various elicitors precursors studied, tryptamine (0.5 mM) precursor bovine serum albumin (BSA) (0.075 an elicitor added on day 6 cultivation period resulted in maximum enhancement concentration (up 4.5 3.4-fold, respectively). These leads provide immense scope for further at bioreactor level. The cytotoxicity analysis crude extract fungal biomass revealed its high effectiveness against colon mammary gland cancer cell lines.
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