Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

摘要: We have established methodology for the isolation and characterization of a novel endophytic fungus from inner bark medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin its related compounds are at present obtained by extraction intact plants, but fungal endopytes may be an alternative source production. In study we observed effect different nutrient combinations precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid leucine) on accumulation Entrophospora infrequens. The were fed either alone or (tryptophan tryptophan acid, leucine). highest content was range 503 ± 25µg/100g dry cell mass medium. medium increased 2.5 folds presence leucine whereas production with trytophan also significantly other treatments. Furthermore, morphology human cancer lines studied. treated cells showed reduction size, condensation nucleus protoplasmic extensions reduced. All these characteristics found apoptotic cells.