Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR
作者： Ewa Pacholewicz , Arno Swart , Len J.A. Lipman , Jaap A. Wagenaar , Arie H. Havelaar
DOI: 10.1016/J.MIMET.2013.06.003
关键词: Broiler 、 Real-time polymerase chain reaction 、 Lari 、 Campylobacter 、 Microbiology 、 Propidium monoazide 、 Biology
摘要: A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, coli and lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The were enumerated by culture for comparison. Campylobacter counts determined PMA-qPCR method not concordant. We conclude that qPCR PMA did fully reduce signal from dead cells.
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