Influencing Factors and Applicability of the Viability EMA-qPCR for a Detection and Quantification of Campylobacter Cells from Water Samples
作者： Diana Seinige , Maren von Köckritz-Blickwede , Carsten Krischek , Günter Klein , Corinna Kehrenberg
DOI: 10.1371/JOURNAL.PONE.0113812
关键词:
摘要: In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection Campylobacter is time consuming and can yield false-negative results, suitability a quantitative real-time PCR method in combination with an ethidium monoazide pretreatment samples (EMA-qPCR) for rapid, viable cells from was investigated. EMA-qPCR has shown to be promising rapid spp. food samples. Application membrane filtration centrifugation, two methods frequently used isolation bacteria water, revealed mean loss up 1.08 log10 cells/ml spiked Both alone lead dead accumulation sample as fluorescence microscopy. After samples, no significant differences could detected subsequent qPCR experiments without EMA compared enumeration. High correlations (R 2 50.942 EMA, R 50.893 EMA) were
harvard.edu
paperity.org
researchgate.net 参考文章(37)
Brian G. Blackburn, Jonathan S. Yoder, Nora Chen, Deborah A. Levy, Sherline H. Lee, Gunther F. Craun, Rebecca L. Calderon, Michael J. Beach, Vincent Hill, Surveillance for waterborne-disease outbreaks associated with recreational water--United States, 2001-2002. Morbidity and Mortality Weekly Report. ,vol. 53, pp. 1- 22 ,(2004)
M.I. Van Dyke, V.K. Morton, N.L. McLellan, P.M. Huck, The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada Journal of Applied Microbiology. ,vol. 109, pp. 1053- 1066 ,(2010) , 10.1111/J.1365-2672.2010.04730.X
J.-P. Butzler, Campylobacter, from obscurity to celebrity Clinical Microbiology and Infection. ,vol. 10, pp. 868- 876 ,(2004) , 10.1111/J.1469-0691.2004.00983.X
Sophie-Madeleine Gubbels, Katrin G Kuhn, Jonas T Larsson, Marianne Adelhardt, Jørgen Engberg, Pernille Ingildsen, Line Wilchen Hollesen, Steen Muchitsch, Kåre Mølbak, Steen Ethelberg, None, A waterborne outbreak with a single clone of Campylobacter jejuni in the Danish town of Køge in May 2010. Scandinavian Journal of Infectious Diseases. ,vol. 44, pp. 586- 594 ,(2012) , 10.3109/00365548.2012.655773
Ingeborg Hein, Wilfried Schneeweiss, Christian Stanek, Martin Wagner, Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR. Journal of Microbiological Methods. ,vol. 71, pp. 336- 339 ,(2007) , 10.1016/J.MIMET.2007.09.005
Ewa Pacholewicz, Arno Swart, Len J.A. Lipman, Jaap A. Wagenaar, Arie H. Havelaar, Birgitta Duim, Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR Journal of Microbiological Methods. ,vol. 95, pp. 32- 38 ,(2013) , 10.1016/J.MIMET.2013.06.003
Beatriz Melero, Luca Cocolin, Kalliopi Rantsiou, Isabel Jaime, Jordi Rovira, Comparison between conventional and qPCR methods for enumerating Campylobacter jejuni in a poultry processing plant Food Microbiology. ,vol. 28, pp. 1353- 1358 ,(2011) , 10.1016/J.FM.2011.06.006
C. Jokinen, T.A. Edge, S. Ho, W. Koning, C. Laing, W. Mauro, D. Medeiros, J. Miller, W. Robertson, E. Taboada, J.E. Thomas, E. Topp, K. Ziebell, V.P.J. Gannon, Molecular subtypes of Campylobacter spp., Salmonella enterica, and Escherichia coli O157:H7 isolated from faecal and surface water samples in the Oldman River watershed, Alberta, Canada. Water Research. ,vol. 45, pp. 1247- 1257 ,(2011) , 10.1016/J.WATRES.2010.10.001
A. Banihashemi, M.I. Dyke, P.M. Huck, Long‐amplicon propidium monoazide‐PCR enumeration assay to detect viable Campylobacter and Salmonella Journal of Applied Microbiology. ,vol. 113, pp. 863- 873 ,(2012) , 10.1111/J.1365-2672.2012.05382.X
Nadine Krämer, Charlotta Löfström, Håkan Vigre, Jeffrey Hoorfar, Cornelia Bunge, Burkhard Malorny, A novel strategy to obtain quantitative data for modelling: combined enrichment and real-time PCR for enumeration of salmonellae from pig carcasses. International Journal of Food Microbiology. ,vol. 145, ,(2011) , 10.1016/J.IJFOODMICRO.2010.08.026