Ssg, a putative glycosyltransferase, functions in lipo- and exopolysaccharide biosynthesis and cell surface-related properties in Pseudomonas alkylphenolia

摘要: In the presence of vaporized p-cresol, Pseudomonas alkylphenolia KL28 forms specialized aerial structures (SAS). A transposon mutant strain (C23) incapable forming mature SAS was isolated. Genetic analysis C23 revealed insertion in a gene (ssg) encoding putative glycosyltransferase, which is homologous to aeruginosa PAO1 PA5001 gene. Deletion ssg caused loss lipopolysaccharide O antigen and altered composition exopolysaccharide. Wild-type produced fucose-, glucose- mannose-rich exopolysaccharide, while exopolysaccharide completely lacked fucose mannose, resulting an with glucose as major component. The showed reduced surface spreading, pellicle biofilm formation, probably due cumulative effect truncation composition. Our results show that involved both biosynthesis thus plays important role cell properties cell–cell interactions P. alkylphenolia.