Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr.

摘要: Abstract A genomic library of Pseudomonas syringae pv. glycinea race 6 DNA was constructed in the mobilizable cosmid vector pLAFR1 and maintained Escherichia coli HB101. Completeness estimated by assaying clones for expression ice-nucleating activity E. coli. Ice-nucleation represented approximately once every 600 clones. Six hundred eighty random were mobilized from plasmid pRK2013 individual conjugations to a 5 strain P. s. glycinea. A single clone (pPg6L3) detected that changed specificity virulent (compatible) avirulent (incompatible) on appropriate soybean cultivars. The also into 1 4 strains glycinea, it conferred these transconjugants same host range incompatibility as wild-type strain. mapped restriction endonucleases, two adjacent EcoRI fragments identified transposon Tn5 mutagenesis be important determining specificity. Southern blot analysis showed are unique not present other races tested. pPg6L3 fluorescens Rhizobium japonicum. However, neither isolates nor harboring elicited hypersensitive reaction leaves.