Differential transactivation potential of Oct1 and Oct2 is determined by additional B cell-specific activities.

摘要: Cell type-specific transcriptional regulation is generally believed to be mediated by sequence-specific transcription factors that are specifically present in the corresponding cells. The interaction of lymphoid-specific Oct2 factor has been thought responsible for B cell-specific activity octamer-containing promoter and enhancer elements. Here we show physiological concentrations do not suffice generate octamer-dependent non-B cell lines. Furthermore, have tested elements lines lack endogenous protein. Our results demonstrate these Oct2-deficient cells ubiquitous Oct1 protein able stimulate promoters a level comparable with normal Oct2-positive However, reporter constructs bearing octamer motif distal position stimulated protein, but require presence Oct2. correlates novel octamer-binding complex containing From conclude contain two different activities: one interacts both mediates proximal second confer function from remote position.