High Glucose Alters Retinal Astrocytes Phenotype through Increased Production of Inflammatory Cytokines and Oxidative Stress

摘要: Astrocytes are macroglial cells that have a crucial role in development of the retinal vasculature and maintenance blood-retina-barrier (BRB). Diabetes affects physiology function vascular including astrocytes (AC) leading to breakdown BRB. However, detailed cellular mechanisms AC dysfunction under high glucose conditions remain unclear. Here we show did not induce apoptosis AC, but instead increased their rate DNA synthesis adhesion extracellular matrix proteins. These alterations were associated with changes intracellular signaling pathways involved cell survival, migration proliferation. High also affected expression inflammatory cytokines activated NF-κB, prevented network formation on Matrigel. In addition, showed attenuation conditions, capillary morphogenesis endothelial Matrigel, was mediated through oxidative stress. Antioxidant proteins heme oxygenase-1 peroxiredoxin-2 levels nuclear localization transcription factor factor-erythroid 2-related factor-2. Together our results demonstrated alter by production stress significant impact proliferation, adhesion, migration.