IFN-gamma/lipopolysaccharide activation of macrophages is associated with protein kinase C-dependent down-modulation of the colony-stimulating factor-1 receptor.

摘要: IFN gamma/LPS treatment increases macrophage tumoricidal and microbicidal activity inhibits CSF-1-induced proliferation. The mechanism underlying the latter effect was investigated in CSF-1-dependent mouse cell line, BAC-1.2F5. IFN-gamma LPS together dramatically reduced total number of CSF-1 receptors (CSF-1R) via selective degradation surface form. Processing transport intracellular CSF-1R to were unaffected. alone had no but significantly enhanced LPS-induced down-regulation. reduction protein kinase C-dependent involved changes serine phosphorylation receptor at different sites. down-modulation by this may be important switching off energy-consuming processes CSF-1R-mediated proliferation chemotaxis activated macrophages.